Study on the role of nano antibacterial materials in orthodontics (a review) / Estudo sobre o papel dos materiais nanoantibacterianos na ortodontia

Nanoparticles (NPs) are insoluble particles with a diameter of fewer than 100 nanometers. Two main methods have been utilized in orthodontic therapy to avoid microbial adherence or enamel demineralization. Certain NPs are included in orthodontic adhesives or acrylic resins (fluorohydroxyapatite, fluorapatite, hydroxyapatite, SiO2, TiO2, silver, nanofillers), and NPs (i.e., a thin layer of nitrogen-doped TiO2 on the bracket surfaces) are coated on the surfaces of orthodontic equipment. Although using NPs in orthodontics may open up modern facilities, prior research looked at antibacterial or physical characteristics for a limited period of time, ranging from one day to several weeks, and the limits of in vitro studies must be understood. The long-term effectiveness of nanotechnology-based orthodontic materials has not yet been conclusively confirmed and needs further study, as well as potential safety concerns (toxic effects) associated with NP size.

Nanopartículas (NPs) são partículas insolúveis com diâmetro inferior a 100 nanômetros. Dois métodos principais têm sido utilizados na terapia ortodôntica para evitar a aderência microbiana ou a desmineralização do esmalte: NPs são incluídas em adesivos ortodônticos ou resinas acrílicas (fluoro-hidroxiapatita, fluorapatita, hidroxiapatita, SiO2, TiO2, prata, nanopreenchimentos) e NPs são revestidas nas superfícies de equipamentos ortodônticos, ou seja, uma camada fina de TiO2 dopado com nitrogênio nas superfícies do braquete. Embora o uso de NPs em ortodontia possa tornar acessível modernos recursos, pesquisas anteriores analisaram as características antibacterianas ou físicas por um período limitado de tempo, variando de 24 horas a várias semanas, por isso devem ser compreendidos os limites dos estudos in vitro. A eficácia de longo prazo de materiais ortodônticos com base em nanotecnologia ainda não foi confirmada de forma conclusiva, o que exige mais estudos, bem como potenciais preocupações de segurança (efeitos tóxicos) associadas ao tamanho da NP.

Study on the role of nano antibacterial materials in orthodontics (a review)

Abstract Nanoparticles (NPs) are insoluble particles with a diameter of fewer than 100 nanometers. Two main methods have been utilized in orthodontic therapy to avoid microbial adherence or enamel demineralization. Certain NPs are included in orthodontic adhesives or acrylic resins (fluorohydroxyapatite, fluorapatite, hydroxyapatite, SiO2, TiO2, silver, nanofillers), and NPs (i.e., a thin layer of nitrogen-doped TiO2 on the bracket surfaces) are coated on the surfaces of orthodontic equipment. Although using NPs in orthodontics may open up modern facilities, prior research looked at antibacterial or physical characteristics for a limited period of time, ranging from one day to several weeks, and the limits of in vitro studies must be understood. The long-term effectiveness of nanotechnology-based orthodontic materials has not yet been conclusively confirmed and needs further study, as well as potential safety concerns (toxic effects) associated with NP size.

Resumo Nanopartículas (NPs) são partículas insolúveis com diâmetro inferior a 100 nanômetros. Dois métodos principais têm sido utilizados na terapia ortodôntica para evitar a aderência microbiana ou a desmineralização do esmalte: NPs são incluídas em adesivos ortodônticos ou resinas acrílicas (fluoro-hidroxiapatita, fluorapatita, hidroxiapatita, SiO2, TiO2, prata, nanopreenchimentos) e NPs são revestidas nas superfícies de equipamentos ortodônticos, ou seja, uma camada fina de TiO2 dopado com nitrogênio nas superfícies do braquete. Embora o uso de NPs em ortodontia possa tornar acessível modernos recursos, pesquisas anteriores analisaram as características antibacterianas ou físicas por um período limitado de tempo, variando de 24 horas a várias semanas, por isso devem ser compreendidos os limites dos estudos in vitro. A eficácia de longo prazo de materiais ortodônticos com base em nanotecnologia ainda não foi confirmada de forma conclusiva, o que exige mais estudos, bem como potenciais preocupações de segurança (efeitos tóxicos) associadas ao tamanho da NP.

Ação clareadora e microbiológica do enxaguante bucal com peróxido de hidrogênio: ensaio clínico / Whitening and microbiological action of mouthwash with hydrogen peroxide: clinical trial

Introdução: os enxaguantes bucais clareadores tem sido muito utilizados, porém sua eficiência e efeitos colaterais trazem questionamentos. Objetivo: este ensaio clínico teve como objetivo avaliar se o enxaguante bucal clareador, contendo peróxido de hidrogênio a 1,5%, apresenta ação clareadora e se há algum efeito secundário na cavidade bucal. Metodologia: foram selecionados 10 voluntários com idade média de 21,5 anos, submetidos a avaliação da cor dos dentes com auxílio do espectrômetro em 3 momentos: inicial; com 15 e com 30 dias de uso do enxaguante. A avaliação dos efeitos colaterais foi realizada a partir da coleta de saliva estimulada em 4 momentos: antes e depois ao primeiro uso do produto, com 15 e com 30 dias, e realizadas as análises laboratoriais: fluxo salivar; pH; quantidade de Streptococcus mutans e de Lactobacillus. A normalidade dos dados foi verificada pelo teste de Shapiro-Wilk, comparação de cor pelo teste t dependente, comparação dos microrganismos pelos testes ANOVA de medidas repetida e Tukey. Resultados: as análises de cor dos dentes não evidenciaram nenhuma alteração significativa em nenhum dos tempos investigados. No fluxo salivar, pH e Lactobacillus não houveram alterações significativas. Na quantidade de Streptococcus mutans notou-se um aumento significativo quando comparado os valores após o primeiro uso e com 30 dias. Conclusão: a solução de enxague bucal contendo peróxido de hidrogênio a 1,5% não apresentou alteração significativa na coloração dos dentes e nenhum efeito colateral significativo na atividade cariogênica de acordo com os testes e períodos avaliados.

Introduction: whitening mouthwashes have been widely used, but their efficiency and side effects raise questions. Objective: this clinical trial aimed to assess whether the bleaching mouthwash, containing 1.5% hydrogen peroxide, has a bleaching action and whether there are any side effects in the oral cavity. Methods: 10 volunteers were selected, with a mean age of 21.5 years, who underwent tooth color evaluation with the aid of a spectrometer in 3 moments: initial; with 15 and 30 days of using the washes. The evaluation of side effects was performed from the collection of stimulated saliva in 4 moments: before and after the first use of the product, at 15 and 30 days, and laboratory analyzes were carried out: salivary flow; pH; the number of Streptococcus mutans and Lactobacillus. Normal distribution was verified with Shapiro-Wilk test, comparisons of color were performed with t-test, comparisons of the microorganisms were performed with repeated measures ANOVA and Tukey tests. Results: the analysis did not show any significant changes in any of the investigated times. There were no significant changes in the salivary flow, pH and Lactobacillus. The number of Streptococcus mutans, was noted a significant increase when comparing the values after the first use and with 30 days. Conclusion: the mouthwash containing 1.5% hydrogen peroxide was not shown any significant alterations in the color teeth. There were not significant collateral effects on the cariogenic activity according to the tests and periods evaluated.

Materiales de restauración dental / Dental restorative materials

Objetivo: Identificar los efectos que producen la utilización de materiales restauradores en los tejidos dentarios luego de utilizarlos como una solución funcional y estética ante los problemas provocados por cualquier tipo de microorganismo es necesario. Método: de tipo bibliográfica mediante una búsqueda de artículos en base PubMed y Scielo. Resultados: Se encontraron 30 artículos, de los cuales solo se pudieron utilizar 15 de ellos, ya que contribuyeron a cumplir con el objetivo de esta investigación y el resto no. Conclusiones: Los materiales dentales son la base fundamental de la odontología restauradora, protésica y de varias especialidades y procedimientos realizados por parte del odontólogo. Por lo tanto, es necesario tener conocimientos de cómo actúa cada uno de estos materiales restaurativos, con base en su estructura interna, para poder ver cuál va a ser su comportamiento físico, mecánico y fisicoquímico en su uso odontológico.

Objective: To identify the effects produced by the use of restorative materials on dental tissues after using them as a functional and esthetic solution to the problems caused by any type of microorganism. Method: bibliographic type by means of a search of articles in PubMed and Scielo databases. Results: 30 articles were found, of which only 15 of them could be used, since they contributed to fulfill the objective of this research and the rest could not. Conclusions: Dental materials are the fundamental basis of restorative dentistry, prosthetic dentistry and various specialties and procedures performed by the dentist. Therefore, it is necessary to have knowledge of how each of these restorative materials acts, based on their internal structure, in order to be able to see what their physical, mechanical and physicochemical behavior will be in their dental use.

Tratamiento ortopédico funcional y mecánico para la corrección de la clase II esqueletal / Functional and mechanical orthopedic treatment for the correction of skeletal class II

Objetivo: Analizar las distintas alternativas de tratamiento basadas en Ortopedia Funcional y Mecánica, su efecto a nivel esqueletal, sus beneficios. Método: Revisión bibliográfica. Resultados: Se seleccionaron 15 artículos de un universo de 45, por cuanto eran los que tenían relación directa con el tema de investigación. Conclusión: Se ha logrado identificar las distintas alternativas de tratamiento con ortopedia funcional o mecánica que se pueden utilizar para el tratamiento de la Clase II esqueletal, corroboradas con resultados que evidencian Su efectividad tanto en los cambios craneofaciales, de tejidos blandos y dentales obtenidos al final de la terapéutica, los autores recomiendan la correcta evaluación de los pacientes, antes de determinar la aparatología a utilizar así cómo aprovechar los estadios tempranos de maduración y crecimiento para que los cambios en el patrón esqueletal sean más representativos, efectivos y alcancen el objetivo de mejorar la relación maxilo-mandibular.

Objective: To analyze the different treatment alternatives based on Functional and Mechanical Orthopedics, their effect at skeletal level, their benefits. Method: Bibliographic review. Results: 15 articles were selected from a universe of 45, as they were those directly related to the research topic. Conclusions: We have been able to identify the different treatment alternatives with functional or mechanical orthopedics that can be used for the treatment of skeletal Class II, corroborated with results that show their effectiveness in craniofacial, soft tissue and dental changes obtained at the end of the therapy, the authors recommend the correct evaluation of the patients before determining the appliances to be used, as well as taking advantage of the early stages of maturation and growth so that the changes in the skeletal pattern are more representative, effective and achieve the objective of improving the maxillomandibular relationship.

Erupción dentaria y actividad de caries: ¿es un factor de riesgo en adolescentes?

Resumen Objetivo: describir el comportamiento, distribución y severidad de las lesiones de caries en las superficies oclusales de los segundos molares permanentes. Relacionarlo a su etapa de erupción y presencia de actividad en el resto de la cavidad bucal. Metodología: Se realizó un estudio descriptivo en una muestra de conveniencia de 100 adolescentes, de ambos sexos, (edad promedio=13,39±0,94 años). Los padres respondieron un cuestionario sobre aspectos socioeconómicos y salud general y los adolescentes sobre dieta y hábitos de higiene. Dos examinadoras calibradas realizaron el examen clínico de acuerdo a los criterios de ICDAS II en todas las superficies erupcionadas, así como el mapeo de la cara oclusal y la etapa de erupción de los segundos molares permanentes. Resultados: la prevalencia de caries fue de un 97% y la extensión (CPOD modificado) de 8,45± 5,10. El 82% de los adolescentes con experiencia de caries presentaba lesiones activas y el 62% de los carioactivos presentaban lesiones únicamente en las caras oclusales de los segundos molares permanentes. Se observó mayor prevalencia de lesiones de caries activas en los estadíos I y II de erupción de los segundos molares permanentes, siendo los sitios más afectados la fosa central y mesial y la fosa central y distal, respectivamente. Conclusiones: La erupción activa de molares significa una etapa de riesgo para las personas que debe ser considerada al momento priorizar y establecer programas de salud bucal.

Resumo Objetivo: descrever o comportamento, distribuição e severiidade das lesões de cárie nas superfícies oclusais dos segundos molares permanentes. Relacionar com seu estágio de erupção e presença de atividade no resto da cavidade oral. Metodologia: Foi realizado um estudo descritivo em uma amostra de conveniência de 100 adolescentes, de ambos os sexos, (média de idade = 13,39 ± 0,94 anos). Os pais responderam a um questionário sobre aspectos socioeconômicos e de saúde geral e os adolescentes sobre alimentação e hábitos de higiene. Dois examinadores calibrados realizaram o exame clínico de acordo com os critérios do ICDAS II em todas as superfícies erupcionadas, além de mapear a superfície oclusal e o estágio de erupção dos segundos molares permanentes. Resultados: a prevalência de cárie foi de 97% e a extensão (CPOD modificado) de 8,45 ± 5,10. 82% dos adolescentes com experiência de cárie tinham lesões ativas e 62% dos carioativos tinham lesões apenas nas superfícies oclusais dos segundos molares permanentes. Maior prevalência de lesões de cárie ativas foi observada nos estágios I e II de erupção dos segundos molares permanentes, sendo os locais mais acometidos a fossa central e mesial e a fossa central e distal, respectivamente. Conclusões: A erupção ativa de molares representa uma etapa de risco para as pessoas que deve ser considerada na priorização e estabelecimento de programas de saúde bucal.

Abstract Objective: to describe the behavior, distribution, and severity of lesions on the occlusal surfaces of the second permanent molars. Relate it to its stage of eruption and presence of activity in the rest of oral cavity. Methodology: A descriptive study was conducted in a convenience sample of 100 adolescents of both sexes (mean age = 13.39 ± 0.94 years). The parents answered a questionnaire on socioeconomic characteristics and general health, and the adolescents, one on food and hygiene habits. Two calibrated examiners recorder caries according to ICDAS II on all the dental surfaces . They also mapped the occlusal surface and eruption stage of the second permanent molars. Results: the prevalence of caries was 97%, and the extension (modified DMFT) was 8.45 ± 5.10. Eighty-two percent of the adolescents with caries experience had active lesions, and 62% of those with caries had lesions only on the occlusal surfaces of the second permanent molars. A higher prevalence of active caries lesions was observed in eruption stages I and II of the second permanent molars. The most affected sites were the central and mesial fossa and the central and distal fossa. Conclusions: Active molar eruption entails a risk stage that should be considered when prioritizing and developing oral health programs.

Performance of ICDAS e-learning program in teaching graduate students on the detection of occlusal caries / Desempenho do programa de aprendizagem eletrônico ICDAS no ensino de alunos de pós-graduação na detecção de cáries oclusais

ABSTRACT Objective: The aim was evaluate the validity and reproducibility of the International Caries Detection and Assessment System (ICDAS) in detecting occlusal caries among graduate students after training using the e-learning program. Methods: A sample of forty-two primary teeth with different ranges of occlusal caries was selected. All teeth were mounted on a wax support and standardized photographs (EOS Rebel XTI, Canon, NY, USA) of the occlusal surfaces were taken. An experienced researcher randomly selected one specific site on the occlusal surface of each tooth to be examined later. Fifteen graduate students with no previous experience in the ICDAS scores completed the ICDAS e-learning program in Portuguese. After the training, visual examination of the occlusal surfaces was taken twice, independently, with a two-week interval. After visual examination, the teeth were longitudinally sectioned 1mm away from the previously selected occlusal site. The teeth were fixed on a wax support and photographed. Histological analysis was performed from these photographs by an experienced researcher to evaluate the depth of demineralization. Results: The Intraclass Correlation Coefficient (ICC) inter-examiner values among all participants varied from 0.54 to 0.96 and intra-examiner ICC reproducibility of the examiners ranges from 0.61 to 0.85, showing good to excellent reproducibility. For detection of enamel and dentine lesions (D1), ICDAS obtained sensitivity 0.835 and specificity 0.756. For dentine lesions (D3), the method revealed an increase in sensitivity but specificity was similar to D1. Conclusion: ICDAS e-learning training program was efficient for the training of caries lesions detection among graduate students.

RESUMO Objetivo: O objetivo foi avaliar a validade e a reprodutibilidade do Sistema Internacional de Detecção e Avaliação de Cárie (ICDAS) na detecção de cárie oclusal entre estudantes de pós-graduação após o treinamento usando o programa de e-learning. Métodos: Foi selecionada uma amostra de 42 dentes decíduos com diferentes faixas de cárie oclusal. Todos os dentes foram montados em um suporte de cera e foram tiradas fotografias padronizadas (EOS Rebel XTI, Canon, NY, EUA) das superfícies oclusais. Um pesquisador experiente selecionou aleatoriamente um local específico na superfície oclusal de cada dente para ser examinado posteriormente. Quinze estudantes de pós-graduação sem experiência anterior com o ICDAS concluíram o programa de e-learning em português. Após o treinamento, o exame visual das superfícies oclusais foi realizado duas vezes, independentemente, com intervalo de duas semanas. Após o exame visual, os dentes foram seccionados longitudinalmente a 1mm do local oclusal previamente selecionado. Os dentes foram fixados em um suporte de cera e fotografados. A análise histológica foi realizada a partir dessas fotografias por um pesquisador experiente para avaliar a profundidade da desmineralização. Resultados: Os valores interexaminadores do Coeficiente de Correlação Intraclasse (ICC) entre todos os participantes variaram de 0,54 a 0,96 e a reprodutibilidade intra-examinador do ICC dos examinadores variou de 0,61 a 0,85, mostrando reprodutibilidade boa a excelente. Para detecção de lesões de esmalte e dentina (D1), o ICDAS obteve sensibilidade 0,835 e especificidade 0,756. Para lesões de dentina (D3), o método revelou um aumento na sensibilidade, mas a especificidade foi semelhante ao D1. Conclusão: O programa de treinamento em e-learning do ICDAS foi eficiente no treinamento de detecção de lesões de cárie em estudantes de pós-graduação.

Research progress on electrode technology for dental plaque pH detection / 口腔疾病防治

@#Plaque pH detection technology can detect the risk of caries and assist in the prevention of caries, with a mature theory and a relatively simple operation. With the increasing demand for clinical caries risk detection technology and the rapid development of microelectrode techniques, there is an increasing variety of types of microelectrodes that can detect the pH of dental plaque, including glass microelectrodes, metal oxide microelectrodes and ion-sensitive field effect transistors. The glass microelectrode was the first microelectrode to be applied in this field, but its structure is weak. Among the various options, the iridium oxide microelectrode has become the most promising caries risk detection electrode in recent years because of its high strength and excellent response. Metal oxide microelectrodes can also effectively compensate for the insufficient strength of glass microelectrodes. With advances in electrode technology, miniaturized, sensitive ion-sensitive field effect transistors have attracted the attention of researchers. Scientists have also recently developed a way to detect the pH of dental plaque with an optical no-contact technique. Optical contactless detection technology will not damage the dental plaque structure, so it has great research and clinical prospects. Future research will further improve the strength and performance of these electrodes on the premise of ensuring miniaturization and achieving noncontact detection.

Influence of caries activity and number of saliva donors: mineral and microbiological responses in a microcosm biofilm model

Abstract Objective this study evaluated the mineral and microbiological response of biofilms originating from different types of saliva inoculum with distinct levels of caries activity. Methodology the biofilms grown over enamel specimens originated from saliva collected from a single donor or five donors with two distinct levels of caries activity (caries-active and caries-free) or from pooling saliva from ten donors (five caries-active and five caries-free). The percentage surface hardness change (%SHC) and microbiological counts served as outcome variables. Results the caries activity of donors did not affect the %SHC values. Inoculum from five donors compared to a single donor showed higher %SHC values (p=0.019). Higher lactobacilli counts were observed when saliva from caries-active donors was used as the inoculum (p=0.017). Pooled saliva from both caries activity levels showed higher mutans streptococci counts (p<0.017). Conclusion Overall, pooled saliva increased the mineral response of the derived biofilms, but all the inoculum conditions formed cariogenic biofilms and caries lesions independently of caries activity.

To investigation the influencing factors of dental caries and caries activity test in children aged 3-5 years in the Yuhua area of Changsha / 中国医师杂志

Objective:To investigate influencing factors of dental caries among 3 to 5-year-old children in the Yuhua District of Changsha by Cariostat detection and provide evidence to prevent caries.Methods:1 032 children aged 3-5 years old from 6 kindergartens in Yuhua District of Changsha were randomly selected for oral health examination. 565 children in 6 large classes, 6 middle classes and 6 small classes were randomly selected from 6 kindergartens were perfected caries activity test (CAT) by Cariostat detection.Results:Among 1 032 children, 626 had caries, the caries rate was 60.66%, the caries was 2.87±3.67, and the caries filling rate was 8.53%. The caries rate of maxillary central incisors was the highest (37.89%), followed by mandibular second primary molars (32.56%) and mandibular first primary molars (32.36%). There was significant difference in caries rate among different age groups (χ 2=9.231, P<0.05). The caries rate of 5-year-old children was the highest (66.50%). 1 032 children were divided into 4 groups according to dmft (decayed, missing, filled teeth) value, and the number of caries free group was the largest; the number of caries in the middle caries group was the least. The number of missing teeth in 1 032 children with caries was 2 959. The number of missing teeth in the high caries group was the most, up to 1 861, accounting for 61.89% of the total number of missing teeth. The analysis of 565 children by CAT showed that the caries rate was different in different CAT grades (χ 2=27.390, P<0.05). The caries rate in the high-risk group was the highest, 90.48%. The CAT value was positively correlated with caries ( r=0.261, P<0.05). Conclusions:The incidence of dental caries in children aged 3-5 years in Yuhua District of Changsha was 60.66%. Cariostat method can truly reflect the situation of caries in children and is helpful to screen the population susceptible to caries in children.

Diagnostic Utilization of Laser Fluorescence for Resin Infiltration in Primary Teeth / 대한소아치과학회지

This study was performed to evaluate clinical use of laser fluorescence (LF) to identify early childhood caries lesions suitable for applying resin infiltration.20 exfoliated primary molars with proximal caries were selected and cut buccolingually cross the central pit for regarding the mesial and distal surfaces respectively. 27 specimens corresponding to ICDAS code 1 and 2 were selected and the LF values were measured. When infiltrant resin was applied, double staining for microscopy detection has done simultaneously. Tooth samples were sliced with 0.7 mm thick. The maximum lesion depth, maximum penetration depth, and average penetration rate were measured from the confocal scanning laser microscope image. Pearson correlation analysis was performed.The intraclass correlation coefficient of LF values shows excellent agreement. LF values had positive correlation with penetration rate, but not lesion depth and penetration depth. Significant correlation between LF readings and penetration rate was verified in deep enamel caries and dentin caries except shallow enamel caries.Infiltrant resin could penetrate with a higher rate and LF values could be increased in more active caries lesions. In assessing radiologically similar caries lesion, laser fluorescence might be useful for identifying caries activity.

Validity of the New Caries Activity Test using Real-Time Polymerase Chain Reaction / 대한소아치과학회지

Periogen is a new caries activity test using real-time polymerase chain reaction. The aim of this study was to assess the validity of Periogen by evaluating the correlation with dmft, dmfts indices and comparing with Cariview and caries risk assessment tool (CAT).83 children under 6 participated in this study. Dmft, dmfts indices and CAT were collected through an examination of oral health status. Plaque samples for Periogen and Cariview were collected and manipulated according to the manufactures' instructions.The correlation coefficient of Periogen, Cariview and CAT with the dmfts index were 0.38, 0.56 and 0.66 in each (p < 0.01). The sensitivity of Periogen, Cariview and CAT were 43%, 76% and 95% and specificity were 80%, 72% and 74% respectively. Area under curve under the receiver operating characteristic curves in each method indicated 0.69, 0.81 and 0.85.CAT and Cariview were more effective in evaluation the risk of dental caries than Periogen so far. To be used Periogen clinically, more improvements for higher validity were needed.

Correlation of Caries Experience and Activity between Mothers and Their Children Using a Colorimetric Test / 대한소아치과학회지

The purpose of this study was to compare the validity of Cariview®, a new colorimetric caries activity test, with CRT® bacteria, a conventional bacterial culture method. In addition, this study assesses the correlation between the dental caries experience and activity between mothers and their children.34 pairs of mothers and their children under the age of 6 years participated in this study with informed consent. After filling out a questionnaire and oral examination, the two caries activity tests above were performed on each subject.In the results, Cariview® scores were statistically significant with children's caries experience (r = 0.598, p <0.01) and showed higher correlation than CRT® bacteria scores. Cariview® scores showed statistically significant correlation with the number of decayed teeth in both mothers and children (p <0.05). In both Cariview® and CRT® bacteria tests, there was no statistically significant correlation between caries experience and caries activity (p > 0.05).Cariview® colorimetric test will be clinically useful for predicting future caries risk and establishing a preventative strategy in pediatric dentistry.

Clinical performance of fluorescence-based methods for detection of occlusal caries lesions in primary teeth

Abstract We aimed to investigate the performance of fluorescence-based methods (FBMs), compared to visual inspection after histological validation, in detecting and assessing the activity status of occlusal carious lesions in primary teeth. One examiner evaluated 50 primary molars close to exfoliation in 24 children. Teeth were assessed using quantitative light-induced fluorescence (QLF) and pen-type laser fluorescence (LFpen). After exfoliation, histological validation was performed. Teeth were cut and sections were evaluated for lesion depth and activity status (after utilization of a pH indicator) under a stereomicroscope. Parameters related to the performance of the methods in detecting caries lesions at two thresholds (initial and dentin lesions) were calculated. Regarding the activity status, lesions were classified into sound+inactive or active, and the area under the ROC curve and the diagnostic odds ratio values of the methods were calculated and compared. Evaluation of red fluorescence using QLF presented higher sensitivity but lower specificity than visual inspection in detecting dentin caries lesions. However, QLF considering different parameters and LFpen had similar performance to that obtained with visual inspection. Regarding activity assessment, all FBMs and visual inspection also presented similar performance. In conclusion, FBMs did not prove advantageous for the detection and activity assessment of occlusal caries lesions in primary molars when compared to visual inspection.

Effect of Repeated Oral Health Education on the Oral Health of Preschool Children / 치위생과학회지

This study aimed to investigate the effect of repeated oral health education on the oral health of preschool children. This study classified the control group of children of 5~6 years of age where the oral health education was conducted one time, and the experiment group where the oral health education was conducted 4 times. The oral health conditions of each group and their risk of developing dental caries were also assessed. No significant difference was observed in the participants in terms of general characteristics, oral health conditions, risk of developing dental caries, and oral health behavior after oral health education. The two groups did not show a significant difference (p<0.001) in dental plaque index before and after oral health education, and showed a significant difference (p<0.001) according to the number of education sessions. Before oral health education, the participants in both groups were brushing their teeth incorrectly. However, after the third session, 56.7% of the participants in the experimental group and 9.4% in the control group used the correct method of tooth brushing. A significant difference was observed between the two groups (p<0.05). In addition, when the tooth-brushing time was compared, the two groups showed a significant difference (p<0.001), and a significant difference was observed in terms of the number of education sessions (p<0.05). In summary, repeated oral health education had significant influences on the dental plaque index, tooth-brushing method, brushing occlusal surfaces, and tooth-brushing time. Therefore, when oral health education is carried out, repeated oral health education in children is more effective than one-time oral health education as reflected in the changes in dental plaque index and oral health behavior. Moreover, oral health education is considered effective in changing the oral health behavior of children when conducted at least 3 times.

Flujo salival y caries radicular en adultos mayores autovalentes / Salivary flow and root caries in the elderly living in the community

Introducción Pese al aparente rol de la saliva como protector de la caries radicular, pocos estudios han estudiado la asociación con el flujo salival. Objetivo El propósito de este estudio fue determinar si un menor flujo salival se relaciona con un aumento en la prevalencia y actividad de la caries radicular en personas mayores autovalentes. Materiales y métodos Se aplicó una encuesta sociodemográfica y se solicitó una muestra de flujo salival no estimulado y estimulado a 332 personas mayores autovalentes. Mediante un examen clínico se obtuvo la presencia y actividad de la caries radicular utilizando los criterios ICDAS y los de Ekstrand, respectivamente. Se calculó el root caries index (RCI) y el porcentaje de caries radiculares activas (% CRA). Los datos fueron analizados con el test t de Student, Anova y Kruskal Wallis, con un nivel de significación de 0,05. Resultados El flujo salival fue menor en mujeres y disminuyó con el aumento de la cantidad de fármacos y enfermedades sistémicas (p < 0,05). Ni el RCI ni el % CRA mostraron diferencias significativas en relación con el flujo salival no estimulado, pese a que ambos fueron levemente mayores en sujetos con un flujo salival estimulado normal (p < 0,05). Conclusión El flujo salival no parece asociarse numéricamente con una mayor presencia o actividad de caries radicular en adultos mayores autovalentes.

Introduction Although the role of saliva in the protection against root caries has been widely controversial, few studies have examined the association with salivary flow. Objective To determine if a decreased salivary flow is related to increased prevalence and activity of root caries in the elderly living in the community. Materials and methods A cross-sectional study was conducted on 332 elderly participants. Subjects were interviewed, completed a sociodemographic questionnaire, were orally examined, and donated a sample from unstimulated and stimulated salivary flow. Clinical examinations were carried out to assess prevalence and activity of root caries using ICDAS criteria. The Root Caries Index (RCI) and percentage of Active Root Caries (ARC) were calculated. Data were analysed using the Student t test, ANOVA and Kruskal Wallis, with a significance level of .05. Results Salivary flow was significantly lower in women and in subjects with high drug consumption and systemic diseases (P < .05). Although neither RCI nor the percentage of ARC differed in relation to unstimulated salivary flow, they were slightly higher in people with normal stimulated salivary flow (P < .05). Conclusion Salivary flow does not appear to be numerically associated with the prevalence or the activity of root caries in independent older adults.

Caries prediction using the caries activity test with a sulfisomidine mixture: a 3-year follow-up study / 대한구강보건학회지

OBJECTIVES: The purpose of this study was to evaluate the prediction validity of the caries activity test with a sulfisomidine mixture (SAHS test). METHODS: This longitudinal follow-up study was conducted for 3 years. The subjects were 155 elementary schoolchildren. Oral examination was performed by examining each tooth surface of the subjects. The number of teeth with new caries lesions was calculated by comparing between the baseline data of the initial oral examination and the results of the second oral examination performed after 3 years. The Dentocult SM test was used as the reference in the analysis of the caries prediction validity of the SAHS test. The items of the validity test for carries prediction were as follows: sensitivity, specificity, predictive value, and likelihood ratio. RESULTS: The correlation between new caries lesions and the SAHS test scores was greater than that between new caries lesions and the Dentocult SM test scores. The receiver-operating analysis revealed that the area under the curve of the SAHS test was higher than that of the Dentocult SM test. The caries prediction validity of the SAHS test (grade 12) was as follows: sensitivity, 0.71-0.70; specificity, 0.60-0.58; positive predictive value, 0.79-0.78; negative predictive value, 0.49 (screening criterion 5). The SAHS test scores were similar to or higher than the scores in the items of the Dentocult SM test. CONCLUSIONS: The SAHS test is considered useful for clinical applications.

THE RELATIONSHIP BETWEEN DENTAL CARIES AND GROWTH INDICES AMONG 3-YEAR-OLD CHILDREN ULAANBAATAR CITY / Шинэ санаа Шинэ нээлт

The aim of this study to assess relationship between the dental caries status and growth indices among 3-year-old children of Ulaanbaatar city, Mongolia. The caries prevalence and mean dmft, dmfs score of all children were 94.3% and 8.3±4.8, 11.1±8.6 (p0.05). Children with high salivary flow rate were 3.2% in central kindergarten and 0% in peripheral. The mean±SD of body weight (p0.05) of boys were 15.2±2.2kg and 97.0±6.0cm, of gils 15.9±1.9kg and 97.6±5.8cm, of caries free children 15.9±1.5kg аnd 97.0±5.4cm, and of children with caries 15.6±2.1kg and 97.4±5.4cm (p>0.05), respectively. Children of peripheral kindergarten were lower by 1,4kg and 3.4cm than central (p<0.001). There were the weak and negative correlation between dental caries and growth indices among 3-year-old children of Ulaanbaatar city, Mongolia.

Validity of caries prediction by caries activity tests using sulfisomidine-containing acidic high sucrose liquid medium / 대한구강보건학회지

OBJECTIVES: The purpose of this study was to evaluate the caries prediction validity and between-observer reliability of caries activity tests using sulfisomidine-containing acidic high sucrose liquid medium (SAHS test). METHODS: This longitudinal follow-up study was done for 1 year. The subjects were 188 elementary school children. The caries experience and caries activity of each of the subjects was examined. We adopted both the SAHS test and Dentocult SM test as caries activity tests. We conducted an ROC analysis on the test data, and thereafter calculated the sensitivity, specificity, predictive value, and Kappa statistics. RESULTS: According to the ROC analysis, it was found that both the SAHS test and Dentocult SM test showed a similar level of AUC (0.5 or higher), which means that both tests have significant caries prediction validity. When the validating criterion was based on 1 or more new caries lesions and the screening criterion was based on a score of 5, it was found that the SAHS test (12 grade) showed a sensitivity value of 0.72-0.73, specificity value of 0.48-0.51, positive predictive value of 0.62-0.63, and negative predictive value of 0.61-0.62, respectively. Based on these 4 values, it was found that the SAHS test had a higher grade of sensitivity and negative predictive value than the Dentocult SM test. However, the latter had a higher grade of specificity and positive predictive value. Despite this, there were no significant differences between both tests. The SAHS test had higher between-observer reliability (kappa value) than the Dentocult SM test (0.90>0.88). CONCLUSIONS: In this study, it was found that both the SAHS test and Dentocult SM test had a similar level of caries prediction validity and between-observer reliability.

Assessing the clinical validity of a new caries activity test using dental plaque acidogenicity / 대한구강보건학회지

OBJECTIVES: This study was aimed at evaluating the clinical usability of a new caries activity test (Cariview(R)), which was based on the acidogenic potential of plaque by evaluating it's correlation with the DMFT index and comparing the results of Cariview(R) with those of previous caries activity tests in an adult group. METHODS: Teeth of 74 subjects were examined, and the DMFT index was calculated according to World Health Organization (WHO) criteria to determine the past caries experience. Caries activity tests were performed according to manufacturer's instructions. For Cariview(R) test, plaque samples were collected from the tooth surface by using a sterile cotton swab and incubated at 37degrees C for 48 h; an indicator was added to observe the color changes according to the plaque acidogenicity. The subjects were classified into three groups (Low, Moderate, High) according to the level of caries risk. The data were assessed using one-way ANOVA to compare the caries experiences of risk groups according to the caries activity test results, and the relationships between the caries activity tests and the DMFT index were evaluated. RESULTS: The overall mean DMFT index was 5.70+/-4.42. There was a significant difference in the DMFT index among the caries risk groups (Low, Moderate, High) according to the Cariview(R) test result (P=0.036). Further, there was a positive correlation between the Cariview(R) score and the DMFT index (r=0.23, P=0.047) and between the Cariview(R) score and the previous caries activity tests score (P<0.01). CONCLUSIONS: The new caries activity test (Cariview(R)) can be easily used at dental clinics and enables precise caries risk assessment.